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Method for determination of fatty acids in bovine colostrum using GC-FID
S Yurchenko1, A Sats1, V Poikalainen1
1Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Department of Food Science and Technology, Kreutzwaldi 62, EE51014 Tartu, Estonia.
Food Chemistry
|July 5, 2016
Summary
Bovine colostrum offers essential fatty acids (EFAs), but their composition is understudied. This research validates a precise method for analyzing EFAs in bovine colostrum, enabling routine dairy product analysis.
Area of Science:
- Animal Science
- Analytical Chemistry
- Food Science
Background:
- Bovine colostrum is a rich source of essential fatty acids (EFAs).
- Limited research exists on the specific EFA composition of bovine colostrum.
- Accurate analytical methods are needed to quantify EFAs in this resource.
Purpose of the Study:
- To develop and validate a reliable method for analyzing fatty acid (FA) composition in bovine colostrum.
- To address the gap in knowledge regarding bovine colostrum's EFA profile.
- To establish a procedure for routine determination of FAs in dairy products.
Main Methods:
- Colostrum samples from Holstein-Friesian cattle were analyzed.
- Fatty acids underwent esterification with boron trifluoride as an acidic catalyst.
- Fatty acid methyl esters (FAMEs) were quantified using Gas Chromatography with Flame Ionization Detection (GC-FID).
Main Results:
- The validated method achieved low limits of detection (LOD: 0.11-0.68 ppm) and quantification (LOQ: 0.37-2.27 ppm) for FAMEs.
- High and consistent recoveries were observed in fortified samples, indicating method accuracy.
- The procedure proved effective for routine analysis of fatty acids in dairy products.
Conclusions:
- A robust and validated method for bovine colostrum fatty acid analysis was established.
- This method facilitates accurate determination of essential fatty acids in bovine colostrum.
- The validated procedure supports routine investigation of fatty acids in various dairy products.
