Related Experiment Video
Updated: Mar 18, 2026

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
Choreographing the Double Strand Break Response: Ubiquitin and SUMO Control of Nuclear Architecture
Shane M Harding1, Roger A Greenberg1
1Departments of Cancer Biology and Pathology, Abramson Family Cancer Research Institute, Basser Research Center for BRCA, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA USA.
Abstract:
The cellular response to DNA double strand breaks (DSBs) is a multifaceted signaling program that centers on post-translational modifications including phosphorylation, ubiquitylation and SUMOylation. In this review we discuss how ubiquitin and SUMO orchestrate the recognition of DSBs and explore how this influences chromatin organization. We discuss functional outcomes of this response including transcriptional silencing and how pre-existing chromatin states may control the DSB response and the maintenance of genomic stability.
Related Concept Videos
Fixing Double-strand Breaks
Fixing Double-strand Breaks
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Homologous Recombination
Regulation of Nuclear Protein Sorting
Restarting Stalled Replication Forks

