Related Experiment Video
Updated: Mar 18, 2026

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators
Published on: May 30, 2014
Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by
Clément Ricard1, Lisa Lamasse2, Alexandre Jaouen3
1Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France; Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France; Service de Pharmacie, Assistance Publique, Hôpitaux de Marseille, Marseille, France; Centre de Recherche en Oncobiologie et Oncopharmacologie, INSERM UMR911 and Aix-Marseille Université, Marseille, France; These authors contributed equally to this work.
Abstract:
Simultaneous imaging of different cell types and structures in the mouse central nervous system (CNS) by intravital two-photon microscopy requires the characterization of fluorophores and advances in approaches to visualize them. We describe the use of a two-photon infrared illumination generated by an optical parametric oscillator (OPO) on quantum-dots 655 (QD655) nanocrystals to improve resolution of the vasculature deeper in the mouse brain both in healthy and pathological conditions. Moreover, QD655 signal can be unmixed from the DsRed2, CFP, EGFP and EYFP fluorescent proteins, which enhances the panel of multi-parametric correlative investigations both in the cortex and the spinal cord.
More Related Videos
Related Concept Videos
Fluorescence and Phosphorescence: Instrumentation
Confocal Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

