Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using
Alireza Molazemhosseini1, Luca Magagnin2, Pasquale Vena3
1Dipartimento Chimica Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Via Mancinelli 7, 20131 Milan, Italy. axm1058@case.edu.
Sensors (Basel, Switzerland)
|July 5, 2016
Summary
A novel electrochemical immunosensor detects glycated hemoglobin (HbA1c) in undiluted serum. This cost-effective, disposable biosensor offers a reliable method for monitoring diabetes management.
Area of Science:
- Electrochemistry
- Biosensors
- Medical Diagnostics
Background:
- Accurate monitoring of glycated hemoglobin (HbA1c) is crucial for effective diabetes management.
- Existing methods for HbA1c detection can be complex, time-consuming, or require sample dilution.
- Development of rapid, sensitive, and cost-effective point-of-care diagnostic tools is needed.
Purpose of the Study:
- To develop a single-use, disposable electrochemical immunosensor for detecting HbA1c.
- To utilize undiluted human serum for HbA1c measurement, simplifying the diagnostic process.
- To establish a cost-effective manufacturing method for the disposable biosensor.
Main Methods:
- Fabrication of a three-electrode electrochemical biosensor on a polyethylene terephthalate (PET) substrate using sputtering and thick-film printing.
- Covalent immobilization of anti-HbA1c antibodies onto gold electrodes via 3-Mercaptopropionic acid (MPA) self-assembled monolayers.
- Detection of HbA1c using differential pulse voltammetry (DPV) by measuring the hindrance effect on ferricyanide/ferrocyanide electron transfer.
Main Results:
- The biosensor demonstrated excellent linearity (R² = 0.999) for HbA1c detection in undiluted human serum within the range of 0.1-0.25 mg/mL.
- Characterization using Electrochemical Impedance Spectroscopy (EIS) and X-ray Photoelectron Spectroscopy (XPS) confirmed successful MPA-SAM formation and antibody immobilization.
- The developed biosensor is suitable for cost-effective, roll-to-roll manufacturing, enabling disposable, single-use applications.
Conclusions:
- A highly sensitive and specific disposable electrochemical immunosensor for HbA1c detection in undiluted human serum has been successfully developed.
- The cost-effective fabrication process makes this biosensor a promising tool for routine in vitro monitoring of HbA1c levels.
- This technology holds significant potential for improving diabetes management and patient outcomes.
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