Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

Non-ohmic Devices

Non-ohmic Devices

In most substances, the current flow is proportional to the voltage applied to it. A simple relationship between the values of current, voltage, and resistance is known as Ohm's law. Nonohmic devices do not exhibit a linear relationship between voltage and current. One such device is the semiconducting circuit element known as a diode. A diode is a circuit device that allows current flow in only one direction.
Consider a simple circuit consisting of a battery, a diode, and a resistor. A...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Online affinity micro free-flow electrophoresis for the continuous monitoring of insulin <i>via</i> a competitive immunoassay.

Lab on a chip·2026

Same author

Identifying and minimizing primary sources of temporal broadening in online affinity micro free-flow electrophoresis.

The Analyst·2025

Same author

Assessing Surface Adsorption in Cyclic Olefin Copolymer Microfluidic Devices Using Two-Dimensional Nano Liquid Chromatography-Micro Free Flow Electrophoresis Separations.

Analytical chemistry·2023

Same author

Fabrication of µFFE Devices in COC via Hot Embossing with a 3D-Printed Master Mold.

Micromachines·2023

Same author

Epidemiological modeling in StochSS Live!

Bioinformatics (Oxford, England)·2021

Same author

Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free-flow electrophoresis devices.

Electrophoresis·2019

Search research articles

Related Experiment Video

Updated: Feb 7, 2026

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

3D Printed Micro Free-Flow Electrophoresis Device.

Sarah K Anciaux¹, Matthew Geiger¹, Michael T Bowser¹

¹Department of Chemistry, University of Minnesota , 207 Pleasant Street SE, Minneapolis, Minnesota 55455, United States.

Analytical Chemistry

|July 6, 2016

Summary

Three-dimensional (3D) printing enables cost-effective fabrication of micro free-flow electrophoresis (μFFE) devices. This 3D-printed μFFE device demonstrates comparable performance to traditional glass devices for complex separations.

More Related Videos

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

A 3D-printed Chamber for Organic Optoelectronic Device Degradation Testing

A 3D-printed Chamber for Organic Optoelectronic Device Degradation Testing

Published on: August 10, 2018

Related Experiment Videos

Last Updated: Feb 7, 2026

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

A 3D-printed Chamber for Organic Optoelectronic Device Degradation Testing

A 3D-printed Chamber for Organic Optoelectronic Device Degradation Testing

Published on: August 10, 2018

Area of Science:

Microfluidics
Additive Manufacturing
Analytical Chemistry

Background:

Traditional microfluidic device fabrication is costly, time-consuming, and limits design flexibility.
Additive manufacturing, or 3D printing, offers a promising alternative with reduced costs and rapid prototyping capabilities.

Purpose of the Study:

To fabricate a micro free-flow electrophoresis (μFFE) device using a low-cost, consumer-grade 3D printer.
To assess the performance and limitations of 3D-printed microfluidic devices compared to traditional glass devices.

Main Methods:

Fabrication of a μFFE device using acrylonitrile-butadiene-styrene (ABS) material on a 3D printer.
Optimization of printing parameters to achieve minimum feature sizes (ridges and valleys).
Surface smoothing using acetone vapor and channel bonding techniques.

Main Results:

Reproducible fabrication of microfluidic features with dimensions as small as 20 μm (height) and 30 μm (width).
Stable stream profiles and comparable separation performance to glass μFFE devices for fluorescent dyes.
Successful separation of myoglobin and cytochrome c using the 3D-printed μFFE device.

Conclusions:

3D printing is a viable and cost-effective method for fabricating functional μFFE devices.
3D-printed μFFE devices offer comparable analytical performance to glass counterparts, with potential for wider accessibility.