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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Mar 18, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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CRISPR: a versatile tool for both forward and reverse genetics research.

Channabasavaiah B Gurumurthy1,2, M'hamed Grati3, Masato Ohtsuka4,5

  • 1Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.

Human Genetics
|July 8, 2016
PubMed
Summary
This summary is machine-generated.

Forward and reverse genetics are key to human genetics research. CRISPR/Cas technology enhances both approaches, accelerating the study of genetic diseases and their causes.

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Area of Science:

  • Human Genetics
  • Molecular Biology
  • Genomics

Background:

  • Forward genetics links mutations to disease etiology.
  • Reverse genetics studies gene function by inducing mutations in model organisms.
  • Traditional methods have limitations in human genetics research.

Purpose of the Study:

  • To provide a perspective on CRISPR-based forward and reverse genetics tools.
  • To discuss the applications of these tools in human genetics.
  • To highlight how CRISPR/Cas technology bridges gaps in current research.

Main Methods:

  • Utilizing CRISPR/Cas as a revolutionary genetic engineering tool.
  • Combining the strengths of forward and reverse genetics.
  • Developing genetically engineered animal models to recapitulate human diseases.

Main Results:

  • CRISPR/Cas technology significantly expedites human genetics research.
  • The integration of CRISPR/Cas enhances the power of both forward and reverse genetics.
  • Genetically engineered models demonstrate causality for identified mutations.

Conclusions:

  • CRISPR/Cas tools offer powerful solutions for human genetics research.
  • These tools accelerate the understanding of disease mechanisms.
  • CRISPR-based approaches are crucial for advancing genetic disease studies.