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Updated: Aug 18, 2026

Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
Published on: March 9, 2013
Observations on the alkaline haematin/detergent complex proposed for measuring haemoglobin concentration
O W van Assendelft1, W G Zijlstra
1Division of Host Factors, Centers for Disease Control, Atlanta, GA.
Abstract:
The "alkaline haematin D-575" method for determining haemoglobin, described by Zander et al. [1984) Clin. Chim. Acta 136, 83-93) was tested. Claims that different non-ionic detergents in the reagent result in identical values for the haemoglobin concentration being measured could not be verified. It could also not be verified that a stable end-product with unique spectral characteristics is always reached within approximately 2 min and that conversion of fetal haemoglobin is faster than that with the haemiglobin cyanide method. Because of the many questions regarding the nature and characteristics of the alkaline haematin/detergent complex or complexes, it is not yet possible to recommend this method for routine haemoglobinometry.
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