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Related Experiment Video

Updated: Mar 18, 2026

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Structural and compositional changes of dissolved organic matter upon solid-phase extraction tracked by multiple

Meilian Chen1, Sunghwan Kim2, Jae-Eun Park1,3

  • 1Department of Environment & Energy, Sejong University, Gwangjin-gu, Seoul, 143-747, Republic of Korea.

Analytical and Bioanalytical Chemistry
|July 9, 2016
PubMed
Summary

Solid-phase extraction (SPE) alters dissolved organic matter (DOM) composition, preferentially removing aromatic structures and biopolymers. Researchers recommend caution when interpreting DOM data after SPE due to these changes.

Keywords:
DOM sourcesEEM-PARAFACFT-ICR-MSMolecular weightSEC-OCDSolid-phase extraction

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Organic Geochemistry

Background:

  • Solid-phase extraction (SPE) using PPL sorbent is common for dissolved organic matter (DOM) analysis.
  • However, the impact of SPE on DOM structure and composition remains poorly understood.

Purpose of the Study:

  • To investigate structural and compositional changes in DOM after PPL-based SPE.
  • To compare DOM characteristics before and after SPE using multiple analytical techniques.

Main Methods:

  • Utilized fluorescence spectroscopy, ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), and size exclusion chromatography with organic carbon detector (SEC-OCD).
  • Analyzed DOM from various sources to assess SPE effects.

Main Results:

  • SPE reduced aromaticity and fluorescent DOM (FDOM), indicating preferential removal of aromatic structures.
  • Low recoveries (1-9%) of biopolymers (polysaccharides, proteins) were observed, suggesting caution with high molecular weight compounds.
  • FT-ICR-MS identified preferential loss of tannins, lignins/CRAM, aliphatics, and heteroatomic formulas, often differing by DOM source.

Conclusions:

  • PPL-based SPE significantly alters DOM composition and molecular weight distribution.
  • Findings highlight the need for careful data interpretation when using SPE for DOM analysis.
  • Source-dependent changes in DOM composition after SPE warrant further investigation.