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Updated: Mar 18, 2026
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
Published on: May 2, 2017
Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles
Marta Czugala1,2, Olga Mykhaylyk3, Philip Böhler4
1Department of Ophthalmology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Aim:
To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs).
Materials & Methods:
Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells.
Results:
Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs.
Conclusion:
Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.
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