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Nisin Z Production by Lactococcus lactis IO-1 Using Xylose as a Carbon Source.

N Chinachoti¹, H Matsusaki¹, K Sonomoto¹

¹a Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University.

Bioscience, Biotechnology, and Biochemistry

|July 10, 2016

Summary

Lactococcus lactis IO-1 efficiently produces nisin Z using xylose, achieving a 20% higher yield than glucose. Calcium chloride further boosted nisin Z activity by 1.5 times.

Keywords:

Lactococcus lactis calcium chloride nisin Z production xylose fermentation

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Published on: October 24, 2016

Area of Science:

Microbiology
Biotechnology
Food Science

Background:

Nisin Z is a crucial bacteriocin with broad-spectrum antimicrobial activity.
Optimizing nisin Z production is vital for its industrial applications in food preservation.
Lactococcus lactis is a key lactic acid bacterium used in food fermentation.

Purpose of the Study:

To investigate the potential of xylose as a carbon source for nisin Z production by Lactococcus lactis IO-1.
To determine the optimal fermentation conditions for maximizing nisin Z yield.
To evaluate the effect of calcium chloride (CaCl2) supplementation on nisin Z production.

Main Methods:

Fermentation of Lactococcus lactis IO-1 using xylose as the primary carbon source.
Optimization of fermentation parameters including substrate concentration, pH, and temperature.
Analysis of nisin Z yield, cell growth, acid production, and sugar consumption.
Assessment of the impact of CaCl2 addition on nisin Z activity.

Main Results:

Xylose supported nisin Z production with a yield 20% superior to glucose.
Optimal conditions identified as 4% xylose, pH 6.0, and 37°C.
Addition of 0.1 M CaCl2 specifically enhanced nisin Z production by 1.5 times without affecting other parameters.

Conclusions:

Xylose is a viable and efficient carbon source for nisin Z production.
Optimized fermentation conditions and CaCl2 supplementation significantly improve nisin Z yield and activity.
This study provides a basis for enhanced industrial-scale nisin Z production.