Related Experiment Video
Updated: Mar 18, 2026

09:02
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Published on: January 8, 2015
17.2K
Effective Cloning of Unmarked DNA Fragments in the Bacillus subtilis 168 Genome
1a Mitsubishi Kasei Institute of Life Sciences.
Bioscience, Biotechnology, and Biochemistry
|July 10, 2016
Abstract:
The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

