Related Experiment Video
Updated: Aug 13, 2026

The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168
Abstract:
Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the theta-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants of B. subtilis. In contrast, linearized pGETS103 gave tetracycline-resistant transformants only when the recipient strain had the pBR322 sequence in the genome. The efficiency and fidelity of the recombinational transfer of DNAs of up to 90 kb are demonstrated.
More Related Videos
Related Concept Videos
Recombinant DNA
Bacterial Transformation
Bacterial Transformation
Mechanism of Conjugation
Transduction

