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Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
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A novel, sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using
Sharmili Roy1, Sim Xiao Wei1, Jean Liew Zhi Ying1
1Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410 Brunei Darussalam.
Biosensors & Bioelectronics
|July 10, 2016
Summary
This study integrates loop-mediated isothermal amplification (LAMP) with electrochemiluminescence (ECL) for sensitive DNA detection. The combined method accurately quantifies DNA from various sources, including pork, at picogram levels.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Biosensing
Background:
- Electrochemiluminescence (ECL) is a sensitive technique for nucleic acid testing.
- Loop-mediated isothermal amplification (LAMP) offers rapid and specific DNA amplification under isothermal conditions.
Purpose of the Study:
- To develop a highly sensitive method for DNA detection and quantification by integrating LAMP with ECL.
- To demonstrate the method's capability for detecting sequence-specific DNA from different meat species and bacterial samples.
Main Methods:
- Integration of LAMP with ECL for DNA detection.
- Electrostatic binding of target LAMP DNA with [Ru(bpy)3](+2) on a carbon electrode.
- ECL reaction triggered by tripropylamine (TPrA) for luminescence generation.
Main Results:
- Detection of Sus scrofa (pork) DNA at levels as low as 1 pg/µL.
- Detection of Bacillus subtilis DNA with a limit of 10 pg/µL.
- Demonstrated high sensitivity and signal amplification through TPrA and LAMP-ECL synergy.
Conclusions:
- The integrated LAMP-ECL method provides a sensitive, isothermal, and robust platform for DNA detection.
- This technique is suitable for multiplex detection and potential application in point-of-care (POC) handheld devices.
Keywords:
Bacillus subtilisElectrochemiluminescenceLoop-mediated isothermal amplificationNucleic acid detectionSus scrofaMore Related Videos
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