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Updated: Mar 18, 2026

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
Published on: June 28, 2019
Synthesis of Didocosahexaenoylphosphatidylserine
R Nakashima1, K Kado1, T Kume1
1a Osaka Laboratory, Chemicals Inspection and Testing Institute , Japan, 1-6-5 Dogashiba, Tennouji-ku, Osaka 543 , Japan.
Abstract:
1,2-Di-O-isopropylideneglycerophosphorochloridate prepared from isopropylindene glycerol and phosphorus oxychloride, was allowed to react with Z-l-serine-N-phthalimidomethyl ester to obtain a derivative of phosphatidylserine. Then, after the isopropylidene group was removed by Amberlite IR-120 (H(+)), and the phosphate group was also blocked as a Ba-salt, this derivative was coupled with docosahexaenoic acid, applying the method of activated ester. Removal of both protective groups of serine was finally done by dry hydrogen chloride in chloroform.
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