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Isolation of Mouse Coronary Endothelial Cells
Published on: July 3, 2016
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Isolation of Mouse Coronary Endothelial Cells
Shizhen Luo1, Aaron H Truong1, Ayako Makino2
1Department of Physiology, University of Arizona, Tucson.
Journal of Visualized Experiments : Jove
|July 13, 2016
Summary
This study presents an efficient method for isolating pure adult mouse coronary endothelial cells. This technique is crucial for studying cardiovascular diseases and endothelial cell function.
Area of Science:
- Cardiovascular Biology
- Cell Biology
- Vascular Biology
Background:
- Endothelial cells are vital for vascular health, regulating tone, permeability, and angiogenesis.
- Endothelial cell dysfunction contributes to cardiovascular diseases like ischemic heart disease.
Purpose of the Study:
- To develop an efficient protocol for isolating high-purity adult mouse coronary endothelial cells.
- To provide a reliable method for obtaining cells for subsequent functional and molecular studies.
Main Methods:
- Adult mouse hearts were dissected and minced.
- Tissue was digested using dispase and collagenase II.
- Cells were isolated using anti-CD31 antibody-conjugated magnetic beads.
Main Results:
- The protocol yields approximately 10^4 cells per heart.
- Isolated cells demonstrated over 90% purity.
- The method is suitable for imaging and molecular biology experiments.
Conclusions:
- This protocol provides an efficient and high-purity method for isolating mouse coronary endothelial cells.
- The isolated cells are suitable for various downstream applications in cardiovascular research.
