Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibody Structure01:10

Antibody Structure

67.1K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
67.1K
Transcytosis of IgG01:15

Transcytosis of IgG

4.4K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
4.4K
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

18.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.3K
Cross-reactivity00:42

Cross-reactivity

33.7K
Overview
33.7K
Allergic Reactions02:06

Allergic Reactions

33.3K
Overview
33.3K
Hypersensitivity Reactions: Immune-Complex Reactions01:19

Hypersensitivity Reactions: Immune-Complex Reactions

96
Type III hypersensitivity reactions occur when antigen–antibody complexes form and activate the complement system. Normally, these complexes help the clearance of antigens by phagocytes and red blood cells. However, when large numbers of immune complexes are present, they can deposit in tissues—particularly in the walls of blood vessels—leading to inflammation and tissue injury. These deposits trigger complement activation and neutrophil recruitment, resulting in serum...
96

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rhinitis associated with asthma is distinct from rhinitis alone: The ARIA-MeDALL hypothesis.

Allergy·2023
Same author

Influence of weather patterns and air quality on ecological population dynamics of ectoparasites in goats.

International journal of biometeorology·2020
Same author

2019 Frank Stinchfield Award: A comparison of prosthetic joint infection rates between direct anterior and non-anterior approach total hip arthroplasty.

The bone & joint journal·2019
Same author

Guidance to 2018 good practice: ARIA digitally-enabled, integrated, person-centred care for rhinitis and asthma.

Clinical and translational allergy·2019
Same author

MASK 2017: ARIA digitally-enabled, integrated, person-centred care for rhinitis and asthma multimorbidity using real-world-evidence.

Clinical and translational allergy·2018
Same author

Working while unwell: Workplace impairment in people with severe asthma.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2018
Same journal

Australasian chronic lymphocytic leukaemia consensus statement: 2026 update.

Internal medicine journal·2026
Same journal

The paradox of peer review: protecting science or policing thought?

Internal medicine journal·2026
Same journal

Immune-related pancytopenia in pregnancy.

Internal medicine journal·2026
Same journal

Crossover effect: causal machine learning reveals opposing mortality responses to mean arterial pressure targets among phenotypically distinct hypertensive patients with septic shock.

Internal medicine journal·2026
Same journal

Clinicopathological findings, correlations and outcomes in patients with renal disease and living with antiretroviral-treated human immunodeficiency virus infection.

Internal medicine journal·2026
Same journal

Approach to thyroid disorders associated with immune checkpoint inhibitors and tyrosine kinase inhibitors.

Internal medicine journal·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

17.0K

Elevated total serum immunoglobulin E (>1000 IU/mL): implications?

T R Tay1, J Bosco1, H Aumann2

  • 1Allergy, Immunology and Respiratory Medicine, Alfred Hospital, Melbourne, Victoria, Australia.

Internal Medicine Journal
|July 14, 2016
PubMed
Summary
This summary is machine-generated.

High immunoglobulin E (IgE) levels (>1000 IU/mL) are often linked to specific conditions. However, many patients with elevated IgE have common allergies like asthma, limiting the test's diagnostic value.

Keywords:
allergic broncho-pulmonary aspergillosisatopyeczemaimmunoglobulin E

More Related Videos

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
06:34

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

10.5K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

4.0K

Related Experiment Videos

Last Updated: Mar 18, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

17.0K
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
06:34

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

10.5K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

4.0K

Area of Science:

  • Immunology
  • Allergy and Clinical Immunology

Background:

  • Elevated total serum immunoglobulin E (IgE) is associated with atopic eczema, allergic bronchopulmonary aspergillosis, helminthic infections, and primary immunodeficiencies.
  • These conditions typically present with IgE levels exceeding 1000 IU/mL.

Purpose of the Study:

  • To investigate the spectrum of conditions associated with markedly elevated total serum IgE levels (>1000 IU/mL).
  • To assess the diagnostic utility of high IgE levels in a clinical setting.

Main Methods:

  • Retrospective analysis of 352 patients with serum IgE >1000 IU/mL over a 5-year period.
  • Review of patient diagnoses and clinical data.

Main Results:

  • Less than 50% of patients with IgE >1000 IU/mL had the classically associated conditions.
  • The majority of remaining patients with markedly elevated IgE were diagnosed with asthma, allergic rhinitis, and food allergy.
  • These common allergic conditions represent scenarios where high IgE testing has limited diagnostic utility.

Conclusions:

  • The diagnostic yield of testing for markedly elevated serum IgE is lower than expected.
  • Common allergic diseases like asthma, allergic rhinitis, and food allergy are frequent findings in patients with high IgE, diminishing its specific diagnostic value in these cases.