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FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

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Fluorescence In Situ Hybridization: Trisomy 12 Follow-up In Hairy Cell Leukemia.

M E Rendal-Vázquez¹, M J Pereira-Leahy¹, J Torea¹

¹a Department of Hematology and Hemotherapy , Juan Canalejo-Teresa Herrera Hospital . La Coruña . Spain .

Hematology (Amsterdam, Netherlands)

|July 14, 2016

Summary

This summary is machine-generated.

Trisomy 12, a chromosomal abnormality, is frequently detected in hairy cell leukemia (HCL) patients using fluorescence in situ hybridization (FISH). This finding suggests trisomy 12 is a valuable marker for HCL presence and disease activity.

Keywords:

FISH HCL Interphase cells Trisomy 12

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Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

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Published on: January 28, 2014

Area of Science:

Hematology
Cytogenetics
Oncology

Background:

Hairy cell leukemia (HCL) is a rare chronic lymphoid leukemia.
Chromosomal abnormalities play a role in leukemia development and progression.
Accurate detection methods are crucial for diagnosis and monitoring.

Purpose of the Study:

To investigate the incidence of trisomy 12 in patients with HCL using fluorescence in situ hybridization (FISH).
To evaluate the utility of trisomy 12 as a biomarker for HCL detection and disease activity.

Main Methods:

Peripheral white blood cells from five HCL patients and 28 healthy controls were analyzed.
Fluorescence in situ hybridization (FISH) with a chromosome 12-specific probe was employed.
Numerical chromosomal abnormalities, specifically trisomy 12, were quantified in interphase and metaphase cells.

Main Results:

Trisomy 12 was detected in three of five HCL patients, including cases of relapse.
The presence of trisomy 12 correlated with HCL activity, often disappearing upon successful treatment.
FISH identified trisomy 12 at a higher frequency than conventional cytogenetic methods.

Conclusions:

Trisomy 12 is a frequent chromosomal abnormality in HCL, detectable by FISH.
Trisomy 12 serves as a potential marker for HCL presence and monitoring disease activity.
FISH offers a sensitive method for detecting trisomy 12 in HCL patients.