Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Newly Recognized CNS Tumors in the 2021 World Health Organization Classification: Imaging Overview with Histopathologic and Genetic Correlation.

AJNR. American journal of neuroradiology·2023
Same author

The representation of authors of color in schizophrenia research articles published in high-impact psychiatric journals.

Schizophrenia research·2022
Same author

A Clinical Study on the Circadian Rhythm of Salivary Cortisol on Aggressive Periodontitis and Its Correlation with Clinical Parameters using Electrochemiluminescence Immunoassay Method.

The journal of contemporary dental practice·2019
Same author

RECENT ADVANCES IN TUBERCULOSIS DIAGNOSTIC TECHNIQUES.

Medical journal, Armed Forces India·2017
Same author

ACTINOMYCETOMA OF HAND AND FOOT.

Medical journal, Armed Forces India·2017
Same author

SURGEON AND HIV INFECTION: Post Exposure Prophylaxis: Need of the hour.

Medical journal, Armed Forces India·2017

Related Experiment Video

Updated: Mar 18, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.3K

A STRATEGY FOR RAPID IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS NASAL CARRIER STATUS.

P K Menon1, A B Desai2, A Nagendra3

  • 1Reader, Medical Cadet, Armed Forces Medical College, Pune - 411 040.

Medical Journal, Armed Forces India
|July 14, 2016
PubMed
Summary

Rapidly identify Methicillin-Resistant Staphylococcus aureus (MRSA) using Mannitol Cloxacillin Salt Agar (MCSA) combined with multiplex PCR. This method accurately detects MRSA nasal carriage, enabling faster infection control in hospitals.

Keywords:
Mannitol cloxacillin salt agarMethicillin Resistant S aureusMethicillin Sensitive Coagulase Negative S aureusMethicillin Sensitive S aureus

More Related Videos

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.4K
Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

5.0K

Related Experiment Videos

Last Updated: Mar 18, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.3K
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.4K
Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

5.0K

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Methicillin-Resistant Staphylococcus aureus (MRSA) poses a significant threat due to its multi-drug resistance and role in severe hospital-acquired infections.
  • Nasal carriage of MRSA among patients, staff, and visitors facilitates its spread within healthcare settings.
  • Current identification methods, like Mannitol Cloxacillin Salt Agar (MCSA), can lack specificity, leading to inaccurate MRSA diagnoses.

Purpose of the Study:

  • To develop and evaluate a rapid and specific method for identifying MRSA nasal carriage.
  • To combine culture-based identification with molecular detection for improved accuracy.
  • To facilitate early implementation of infection control measures by enabling rapid screening.

Main Methods:

  • Anterior nasal swabs were collected from hospital patients and nursing staff.
  • Samples were cultured on Mannitol Cloxacillin Salt Agar (MCSA).
  • Multiplex PCR targeting the mecA and femB genes was performed on isolates showing growth and color change.

Main Results:

  • Of 100 inoculated tubes, 8 showed growth and color change. Conventional testing identified 4 MRSA, 3 MSSA, and 1 MSCNS.
  • Multiplex PCR genotyping revealed 5 MRSA, 2 MSSA, and 1 MRCNS.
  • MRSA isolates consistently showed both mecA and femB genes, while methicillin-sensitive strains lacked mecA and coagulase-negative strains lacked femB.

Conclusions:

  • Combining MCSA with multiplex PCR for mecA and femB genes provides a rapid and specific method for MRSA identification.
  • This strategy allows for efficient screening of nasal carriers.
  • Early identification of MRSA carriers can lead to prompt implementation of hospital infection control protocols.