Microarray dataset of Jurkat cells following miR-93 over-expression

Area of Science:

• Molecular Biology
• Genomics
• Gene Expression Analysis

Background:

• MicroRNAs (miRNAs) play crucial roles in gene regulation.
• Dysregulation of miRNAs is implicated in various cellular processes and diseases.
• Jurkat cells are a widely used human T-cell leukemia line for immunological and molecular studies.

Purpose of the Study:

• To investigate the global gene expression changes in Jurkat cells upon over-expression of miR-93.
• To provide a comprehensive dataset of microarray analysis for miR-93 over-expression.
• To identify potential target genes and pathways regulated by miR-93 in this cell line.

Main Methods:

• Microarray hybridization was performed on Jurkat cells engineered to over-express miR-93 using lentiviral transduction.
• Three biological replicates were utilized to ensure data robustness.
• Data normalization and processing were conducted, followed by differential gene expression analysis (log2 Fold Change).

Main Results:

• A ranked list of differentially expressed genes based on log2 Fold Change was generated.
• Gene Ontology (GO) term enrichment analysis was performed to identify affected biological pathways.
• Normalized and processed microarray data are available for further research.

Conclusions:

• Over-expression of miR-93 in Jurkat cells leads to significant alterations in global gene expression patterns.
• The provided dataset and analysis offer insights into miR-93-mediated regulatory networks.
• This resource facilitates further investigation into the functional roles of miR-93 in T-cell biology.