Bacteria-Templated NiO Nanoparticles/Microstructure for an Enzymeless Glucose Sensor.

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Green Chemistry

Background:

• Enzyme-free glucose detection is crucial for developing reliable biosensors.
• Nickel oxide (NiO) nanomaterials offer promising electrocatalytic properties.
• Bacterial synthesis provides a sustainable route for nanomaterial production.

Purpose of the Study:

• To develop and characterize a bacterial-induced hollow cylinder NiO (HCNiO) nanomaterial for glucose sensing.
• To evaluate the electrocatalytic performance of HCNiO for glucose oxidation in basic media.
• To establish the fundamental electrochemical parameters and analytical performance of the HCNiO-based glucose sensor.

Main Methods:

• Synthesis of HCNiO nanomaterial using a bacterial template.
• Electrochemical characterization using cyclic voltammetry (CV) and amperometry (i-t).
• Analysis of electrochemical parameters including diffusion coefficient, heterogeneous rate constant, and electroactive surface coverage.

Main Results:

• HCNiO demonstrated efficient electrocatalytic oxidation of glucose in 0.05 M NaOH.
• The sensor exhibited two linear ranges for glucose detection (0.2–3.5 µM and 0.5–250 µM).
• High sensitivity (3978.9 µA mM⁻¹ cm⁻²) and a low limit of detection (0.9 µM) were achieved.

Conclusions:

• Bacterial-induced HCNiO is a viable material for sensitive, enzyme-free glucose detection.
• The Ni(2+)/Ni(3+) redox couple on the HCNiO electrode facilitates glucose oxidation.
• This green synthesis approach offers a promising platform for developing advanced glucose sensors.