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Multifluorophore localization as a percolation problem: limits to density and precision.

Journal of the Optical Society of America. A, Optics, Image Science, and Vision

|July 14, 2016

Summary

This summary is machine-generated.

Determining optimal fluorophore density in super-resolution microscopy is key. Exceeding this density, analyzed via percolation theory, compromises localization accuracy and precision.

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Area of Science:

Optics and Photonics
Biophysics
Physical Chemistry

Background:

Super-resolution microscopy enables imaging beyond the diffraction limit.
High fluorophore density is crucial for dense imaging but can lead to signal overlap.
Understanding the limits of fluorophore density is essential for optimizing imaging performance.

Purpose of the Study:

To determine the maximum desirable density of activated fluorophores in super-resolution experiments.
To establish a theoretical framework for predicting optimal fluorophore density.
To relate density limits to imaging parameters like localization accuracy and photon emission.

Main Methods:

Applying percolation theory to model overlapping point spread functions.
Deriving a theoretical bound for activated fluorophore density.
Considering factors such as localization accuracy, precision, and photon counts.

Main Results:

A theoretical bound for the maximum desirable density of activated fluorophores was derived.
The derived bound aligns closely with experimentally observed densities.
The study quantifies the trade-off between fluorophore density and localization performance.

Conclusions:

Percolation theory provides a framework for understanding density limits in super-resolution microscopy.
Exceeding the derived density bound will likely reduce localization accuracy and precision.
Further increases in fluorophore density necessitate careful consideration of imaging parameter trade-offs.