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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Mar 18, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Single molecule image formation, reconstruction and processing: introduction.

Amit Ashok, Rafael Piestun, Sjoerd Stallinga

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |July 14, 2016
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    Summary
    This summary is machine-generated.

    Single molecule imaging advances molecular biology research by exploring fundamental limits and novel techniques. This collection offers new insights into image formation and analysis for enhanced scientific discovery.

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    Area of Science:

    • Molecular biology
    • Biophysics
    • Microscopy

    Background:

    • Single molecule imaging has transformed molecular biology research.
    • Understanding the fundamental limits of imaging is crucial for scientific advancement.

    Discussion:

    • This feature issue compiles research on the frontiers of single molecule imaging.
    • It highlights innovative methods for image formation and data analysis.

    Key Insights:

    • New insights into the theoretical and practical limitations of imaging single molecules.
    • Novel techniques are presented for improved image resolution and accuracy.

    Outlook:

    • Future directions in single molecule imaging technology.
    • Potential applications in understanding complex biological processes at the molecular level.