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[Diagnostic values of plasma CD64 and sTREM-1 for pediatric pneumonia]
Mei-Feng Zhong1, Jian-Mei Zhao
1Department of Pediatrics, Huai'an Huaiyin Hospital, Huai'an, Jiangsu 223300, China. 2687084388@qq.com.
Insights
Plasma CD64 and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) show promise in diagnosing pediatric bacterial pneumonia. Combining these biomarkers offers improved diagnostic accuracy for bacterial pneumonia in children.
Area of Science:
- Pediatric infectious diseases
- Immunology
- Biomarker research
Background:
- Pneumonia is a leading cause of childhood mortality globally.
- Accurate differentiation between bacterial and non-bacterial pneumonia is crucial for effective treatment.
- Novel diagnostic markers are needed to improve early and precise diagnosis in pediatric pneumonia.
Purpose of the Study:
- To evaluate the diagnostic utility of plasma CD64 and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in children with pneumonia.
- To compare the diagnostic performance of CD64 and sTREM-1 individually and in combination for bacterial pneumonia.
Main Methods:
- Sixty children with pneumonia were categorized into bacterial, viral, and Mycoplasma pneumonia groups.
- Thirty healthy children served as the control group.
- Plasma concentrations of CD64 and sTREM-1 were measured using ELISA, and diagnostic accuracy was assessed via ROC curve analysis.
Main Results:
- Plasma CD64 and sTREM-1 levels were significantly elevated in the bacterial pneumonia group compared to other groups.
- The combination of CD64 and sTREM-1 demonstrated superior diagnostic performance (AUC=0.956) compared to individual markers.
- Combined markers achieved 93.15% sensitivity and 91.54% specificity for bacterial pneumonia.
Conclusions:
- Plasma CD64 and sTREM-1 are valuable biomarkers for diagnosing pediatric bacterial pneumonia.
- The combined use of CD64 and sTREM-1 enhances diagnostic accuracy, aiding in clinical decision-making for pediatric pneumonia cases.
Objective:
To determine the diagnostic values of plasma CD64 and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in children with pneumonia.
Methods:
Sixty children with pneumonia between August 2014 and October 2015 were classified into bacterial pneumonia group (25 cases), viral pneumonia group (17 cases), and Mycoplasma pneumonia group (18 cases) according to their clinical manifestations, pathogen cultures, and X-ray findings. Another 30 healthy children who underwent physical examination during the same period were selected as the control group. The concentrations of CD64 and sTREM-1 in blood samples were determined using ELISA. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic sensitivity and specificity of plasma CD64 and/or sTREM-1 for bacterial pneumonia.
Results:
The expression of CD64 and sTREM-1 in the bacterial pneumonia group was significantly higher than that in the viral pneumonia, Mycoplasma pneumonia, and control groups (P<0.05). The areas under the ROC curves of CD64, sTREM-1, and a combination of the two markers for diagnosing bacterial pneumonia were 0.878, 0.805, and 0.956, respectively. The sensitivity and specificity of CD64 for diagnosing bacterial pneumonia were 81.30% and 92.32%, respectively, when the cut-off value was 641 pg/mL. The sensitivity and specificity of sTREM-1 for diagnosing bacterial pneumonia were 78.65% and 84.67%, respectively, when the cut-off value was 1 479 pg/mL. The sensitivity and specificity of a combination of the two markers for diagnosing bacterial pneumonia were 93.15% and 91.54%, respectively.
Conclusions:
Plasma CD64 and sTREM-1 can be used as markers for diagnosing pediatric bacterial pneumonia, and a combination of the two markers results in better diagnosis.
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