Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

15.0K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
15.0K
Pore Transport and Ion-Pair Transport01:17

Pore Transport and Ion-Pair Transport

1.5K
Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited  but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
1.5K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

11.3K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
11.3K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

10.3K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
10.3K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

65.9K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
65.9K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

19.8K
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ion-Pair Breakers and Anionic Brønsted Acids for Helmholtz-Layer Catalysis with External Electric Fields in Microfluidic Capacitors.

JACS Au·2026
Same author

Flipper dendrimers.

Chemical science·2026
Same author

Grafting Cell-Penetrating Poly(disulfide)s to Substrates of Interest: Dynamic Covalent Bioconjugation for Traceless Delivery.

Angewandte Chemie (International ed. in English)·2025
Same author

Thiol-mediated uptake of phosphorothioate liposomes, visualized with fluorescent flippers.

Chemical science·2025
Same author

Organocatalytic Microfluidic Double-Layer Capacitors.

Angewandte Chemie (International ed. in English)·2025
Same author

Translational Supramolecular Thioorthoester Chemistry.

Angewandte Chemie (International ed. in English)·2025
Same journal

From Fundamental Photophysics to Photocatalysis: Energy Gap Law Analysis of Anion Radical Excited States.

ACS central science·2026
Same journal

Mechanical Taming of Hardy-Cope Rearrangements.

ACS central science·2026
Same journal

Validation of <i>De Novo</i> Designs of Solid-Binding Peptides.

ACS central science·2026
Same journal

These Graphene Experts Are Trying to Close the Reproducibility Gap in Two-Dimensional Materials Research.

ACS central science·2026
Same journal

How to Make a Creamy, Tasty Vegan Camembert.

ACS central science·2026
Same journal

Versatile Pyridinium Trifluoroborate Platform for Facile Preparation of <sup>18</sup>F‑Labeled PET Tracers in Water.

ACS central science·2026
See all related articles

Related Experiment Video

Updated: Mar 17, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

21.2K

Anion-π Enzymes.

Yoann Cotelle1, Vincent Lebrun2, Naomi Sakai1

  • 1National Centre of Competence in Research (NCCR) Molecular Systems Engineering, Basel, Switzerland; Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.

ACS Central Science
|July 15, 2016
PubMed
Summary
This summary is machine-generated.

Artificial enzymes utilizing novel anion-π interactions achieve high stereoselectivity in a challenging reaction. This breakthrough rivals conventional catalysts and demonstrates the power of engineered protein environments for catalysis.

More Related Videos

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.7K
A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

608

Related Experiment Videos

Last Updated: Mar 17, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

21.2K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.7K
A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

608

Area of Science:

  • Biocatalysis
  • Organic Chemistry
  • Protein Engineering

Background:

  • Anion-π interactions, a novel interaction class, can stabilize anionic intermediates.
  • Previous artificial catalysts showed poor stereoselectivity in relevant reactions.

Purpose of the Study:

  • To develop stereoselective artificial enzymes using anion-π interactions.
  • To investigate the role of protein environments in enhancing catalytic performance.

Main Methods:

  • Design and synthesis of artificial enzymes with anion-π functionalities.
  • Screening of biotinylated catalysts against streptavidin mutants.
  • Enzyme kinetics and inhibition studies to probe catalytic mechanisms.

Main Results:

  • Engineered S112Y streptavidin mutant achieved 95% enantiomeric excess (ee).
  • Complete suppression of the undesired decarboxylation side product.
  • Anion-π enzyme performance matches or exceeds state-of-the-art organocatalysts.

Conclusions:

  • Anion-π interactions are effective within protein scaffolds for stereoselective catalysis.
  • Protein engineering can fine-tune catalytic activity and selectivity through specific mutations.
  • Selective transition-state stabilization is key to enhanced enantioselectivity and reaction rates.