[Absorption of C14-labeled dye "active red 5CX"++ immobilized on polysaccharides in the rat gastrointestinal tract]

Rats were maintained on a diet containing 14C-aniline procion dye "active red 5CX" either in a hydrolyzed or chemically immobilized form on starch and cellulose. Absorption of the label, distribution in tissues and excretion were studied within 6-48 hrs. Independently on the forms of the preparation used more than 80% of the label occurred in blood and were then excreted with urine. Molecule of the azo-dye appears to degrade in large intestine by means of microflora with splitting of the azo-bond and the potentially toxic metabolites developed penetrated into a body media. Immobilization of the dye on polymers, including cellulose was not an effective approach to prevent these reactions.