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Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney
Published on: August 9, 2014
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Fluorescence-Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish
Thomas J D Bates1, Uta Naumann1, Christoph Englert2,3
1Leibniz Institute for Age -Fritz Lipmann Institute, Beutenbergstrasse 11, 07745, Jena, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2016
Abstract:
Zebrafish is becoming a very important model for studying human diseases. The conserved structure of the nephrons in the kidney allows the user to answer questions relating to study human kidney disorders. Wt1a-expressing podocytes are the most important cells within the glomeruli of adult zebrafish. In order to understand the molecular characteristics of these cells, within damage models, we have established a method for isolating them.
