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Updated: Mar 17, 2026

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
Published on: June 28, 2013
Interactomic landscape of PA-X-chicken protein complexes of H5N1 influenza A virus
Qinghe Li1, Xiaoya Yuan2, Qiao Wang1
1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P, .R. China.
Unlabelled:
As a newly identified isoform generated from the PA segment and an essential factor for viral virulence, little is known about PA-X-host interactions. Here we present the interactomic landscape of PA-X protein of H5N1 influenza A virus (IAV), described using data generated from affinity purification and mass spectrometry (AP-MS). PA-X was exogenously expressed in chicken fibroblast cells and PA-X associated protein complexes were identified by AP-MS. Using a high confidence threshold for interaction 56 unique proteins were found to have physical interactions with PA-X. PA-X associated host factors showed strong enrichment for specific protein domains, including annexin and WD40 domains. Many proteins that have been described as pro or antiviral host proteins interact with PA-X, indicating the possible effect of these proteins on influenza A viral infections facilitated by interactions with PA-X. This study has uncovered the comprehensive interactomic landscape of PA-X and laid the foundation for further understanding of PA-X function in terms of viral-host protein interactions.
Biological Significance:
Identification of viral-host interacting proteins is vital for the comprehensive understanding of how virus recruits the host cellular machinery and how host antagonizes virus infection. Our study reveals the viral-host interactome of PA-X and uncovers interactions between host proteins and PA-X which might have crucial roles in viral infection.
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