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Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
Published on: August 8, 2017
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Transcriptome analysis of sika deer in China
Bo-Yin Jia1, Heng-Xing Ba1, Gui-Wu Wang1
1State Key Laboratory for Molecular Biology of Special Economical Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
Molecular Genetics and Genomics : MGG
|July 18, 2016
Summary
This study sequenced the sika deer transcriptome, identifying tissue-specific genes and developmental patterns. Findings reveal key genetic insights into sika deer growth and development.
Area of Science:
- Genomics
- Transcriptomics
- Animal Science
Background:
- Sika deer (Cervus nippon) possess significant commercial value for their antlers and meat.
- Understanding the molecular basis of sika deer growth and development is crucial for their effective management and utilization.
Purpose of the Study:
- To generate comprehensive transcript sequences for sika deer.
- To identify tissue-specific and age-dependent differential gene expression patterns.
- To establish a functional genomics resource for sika deer research.
Main Methods:
- De novo transcriptome assembly and profiling across ten tissue types and four developmental stages (juvenile, adolescent, adult, aged).
- Utilized Illumina paired-end tag (PET) sequencing technology.
- Annotation of unigenes against public protein databases.
Main Results:
- Generated over 1.7 million contigs and 1.3 million unigenes, with 33.2% annotated.
- Identified numerous tissue-specific and age-dependent unigenes, with testes showing the highest enrichment.
- The juvenile-adolescent transition emerged as a critical developmental stage.
Conclusions:
- This study provides the first multi-tissue, multi-stage transcriptome analysis of sika deer.
- The generated data serve as a valuable resource for functional genomics and understanding sika deer biology.
- Findings can guide gene selection for enhancing growth and development in sika deer.

