[High performance liquid chromatography of phospholipids on modified silica gel]

A sensitive method for the separation of phospholipids by a HPLC procedure is described. The separation was carried out on a 30 cm prepacked column with aminopropyl bonded silica under isocratic conditions. The effective separation of major phospholipid classes was carried out for less than 5 min. These chromatographic conditions were used for analysis of natural phospholipid mixtures.