In Vitro Characterization of TiO2 Nanodots Films on Ti Substrates

In this study, TiO2 nanodots films were prepared on titanium substrates via a sol-gel phase-separation-induced self-assembly method. The in vitro behavior of the films was characterized and discussed. The results indicated that films consisted of individual, polycrystalline TiO2 nanodots were obtained. It was found that the nanodots films showed excellent collagen deposition properties, and the ultraviolet illumination could even improve such deposition. Osteoblastic cells can well attach and spread on the collagen-deposited TiO2 nanodots films. MTS assay revealed that TiO2 nanodots films could promote cell adhesion and proliferation compared with bare titanium substrate. These results indicated that such TiO2 nanodots films could be potentially applied as osseointegration coatings.