Alteration of Cell Morphology with Nano- and Micro-Topographical Surface on Closed-Packed Silica Nanobeads

The nanotopological cues are emerging field of in vivo study, because it stimulates alteration of cell morphology and cell behavior such as adhesion, proliferation, differentiation, apoptosis and migration. However, it has not studies nanosurface affected cancer cell behavior, therefore, in this study, we determined that effects the silica nanobeads used as nanotopological tools on cancer cells. For synthesis of silica beads, we made it using stober method and basic amino acid (L-arginine) instead of NH4OH. We carried out rubbing beads to obtain the monolayer silica beads and it used as nanotopological cues for fabrication. It was induced changing cell morphology at 1DIV in group-II (SB-450 and SB-570). However, it was maintained in group-I (SB-118, SB-230) and group-III (SB-1450) like control. Therefore, we separated type-I and type-II surface along with area of cell adhesion and morphology. The characteristic of type-II surface was long distance between contact points, resulting in increase of tension to cells. We found that the morphology rounded up by type-II surface at 1DIV. We described that the nanosurface-induced mechanical tension is associated with alteration of morphology, thus the silica nanobeads used as nanotopological tools for cancer research.