Related Experiment Video
Updated: Mar 17, 2026
08:31
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
Published on: July 29, 2021
2.0K
An Expedient Synthesis of Fluorescent Labeled Ceramide-1-phosphate Analogues
I A Boldyrev1, R E Brown2, J G Molotkovsky1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russian.
Russian Journal of Bioorganic Chemistry
|July 19, 2016
Abstract:
A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a-diaza-s-indacene-8-yl (Me4-BODIPY) fluorophore at co-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D from Streptomyces chromofuscus; the enzymatic yield has been raised to 50-70% by appliance of organic solvent in the incubation medium.
