Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Renin substrate in human amniotic fluid.

K Metsärinne1, F Fyhrquist, K Rosenlöf

  • 1Minerva Institute for Medical Research, Helsinki University Central Hospital, Finland.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|April 14, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lowering Albuminuria-Does It Lower the Cardiovascular Risk?: Reduction in Albuminuria Translates to Reduction in Cardiovascular Events in Hypertensive Patients: Losartan Intervention for Endpoint Reduction in Hypertension Study. Hypertension 45: 198-202, 2005.

Journal of the American Society of Nephrology : JASN·2023
Same author

Reducing Microalbuminuria-Does It Lower Cardiovascular Risk?: Reduction in Albuminuria Translates to Reduction in Cardiovascular Events in Hypertensive Patients: Losartan Intervention for Endpoint Reduction in Hypertension Study. Hypertension 45: 198-202, 2005.

Journal of the American Society of Nephrology : JASN·2023
Same author

Telomere length and cardiovascular risk in hypertensive patients with left ventricular hypertrophy: the LIFE study.

Journal of human hypertension·2011
Same author

The effect of revascularization of atherosclerotic renal artery stenosis on coronary flow reserve and peripheral endothelial function.

Nephron. Clinical practice·2011
Same author

Sjögren's Syndrome Associated with Vitamin B(12) Deficiency.

Scandinavian journal of rheumatology·2010
Same author

Telomere length and progression of diabetic nephropathy in patients with type 1 diabetes.

Journal of internal medicine·2009

Human amniotic fluid renin substrate (RS) was compared to plasma RS, revealing similar physical and immunological properties. However, amniotic fluid RS exhibited lower immunoreactivity, suggesting biochemical differences.

Area of Science:

  • Biochemistry
  • Reproductive Biology
  • Protein Chemistry

Background:

  • Renin substrate (RS) is a key component of the renin-angiotensin system.
  • Understanding RS in different physiological fluids is crucial for studying its role in blood pressure regulation.
  • Human amniotic fluid represents a unique biological environment with potential differences in protein composition.

Purpose of the Study:

  • To compare purified human amniotic fluid renin substrate (RS) with purified plasma RS.
  • To investigate the biochemical and immunological properties of amniotic fluid RS.
  • To assess the immunoreactivity of amniotic fluid RS in comparison to plasma RS.

Main Methods:

  • Purification of human amniotic fluid and plasma renin substrate.

Related Experiment Videos

  • Comparison of molecular weight, isoelectric point, and immunological properties.
  • Radioimmunoassay (direct and indirect) to measure RS levels and assess immunoreactivity.
  • Analysis of RS in decidual and placental cytosolic fractions.

    • Main Results:

    • Purified amniotic fluid RS and plasma RS showed similar molecular weight, isoelectric point, and immunological characteristics.
    • Radio-iodinated amniotic fluid RS demonstrated lower immunoreactivity compared to plasma RS.
    • Direct radioimmunoassay revealed RS levels in amniotic fluid to be only 10-22% of those measured by indirect assay.
    • No significant difference in immunoreactivity was observed for RS in decidual and placental cytosolic fractions.

    Conclusions:

    • Human amniotic fluid RS possesses biochemical alterations or forms complexes, leading to reduced immunoreactivity compared to plasma RS.
    • These findings suggest a distinct nature of RS in the amniotic fluid environment.
    • Further research is warranted to elucidate the precise biochemical modifications and functional implications of amniotic fluid RS.