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Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

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Updated: Mar 17, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Cationic Lipid-Based Nucleic Acid Vectors.

Emile Jubeli¹, William P D Goldring², Michael D Pungente³

¹Faculté de pharmacie, EA 401, IFR 141, Université Paris-Sud, 92296, Châtenay Malabry, France.

Methods in Molecular Biology (Clifton, N.J.)

|July 21, 2016

Summary

This summary is machine-generated.

Nonviral gene delivery using cationic lipids offers a safer alternative to viral vectors. This chapter details protocols for formulating and assessing cationic lipid-DNA complexes for efficient cellular delivery.

Keywords:

Cationic lipids Charge ratio Co-lipids Cytotoxicity Lipoplexes Nucleic acid delivery Particle size Transfection efficiency

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Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Nucleic acid delivery is crucial for cell culture and gene therapy.
Viral vectors are efficient but costly and carry risks.
Nonviral strategies, particularly cationic lipids, are attractive alternatives.

Purpose of the Study:

To provide detailed protocols for in vitro cationic lipid-based DNA delivery.
To address challenges in developing robust lipoplex formulations.
To facilitate reproducible assessment of nonviral gene delivery systems.

Main Methods:

Formulation of cationic lipid-DNA complexes (lipoplexes).
In vitro cell culture assays for delivery assessment.
Evaluation of cellular uptake and gene expression/silencing.

Main Results:

Established reproducible protocols for lipoplex formulation.
Demonstrated effective in vitro DNA delivery using cationic lipids.
Provided a framework for assessing nonviral vector performance.

Conclusions:

Cationic lipid-based delivery is a viable nonviral strategy for nucleic acid delivery.
Standardized protocols are essential for advancing lipoplex technology.
Further optimization is needed to overcome existing limitations in nonviral gene delivery.