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Published on: July 31, 2015
Application of Polyethylenimine-Grafted Silicon Nanowire Arrays for Gene Transfection
Hongwei Wang1, Jingjing Pan2, Hong Chen2
1Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, P. R. China. wanghw@suda.edu.cn.
Abstract:
Polyplexes are one of the most important and promising approaches to deliver exogenous DNA into cells. However, it is severely restricted by the aggregation of polyplexes. Surface-tethered polyplexes can inhibit the aggregation effect and increase the local concentrations of DNA, exhibiting an excellent potential in gene transfection. Since silicon nanowires have the ability to penetrate the cell membrane, branched polyethylenimine (bPEI)-grafted silicon nanowire arrays (SiNWAs) can stimulate gene transfection to a great extent. Herein, the method for the preparation of bPEI-grafted SiNWAs, as an example of surface-tethered polyplexes, is introduced in detail.
