Evaluation and control of isoflurane during experimental dental procedures on rodents

The purpose of this study was to assess exposures to isoflurane to employees performing an experimental dental procedure on rodents. Isoflurane was used as an anesthetic for rodents during injections and placement of dental apparatus into the mouth. We collected eight full-shift samples using passive badges over a three-week period. The procedure was performed once a week for approximately 45 min each day. Results showed that employees were being exposed to isoflurane above the National Institute for Occupational Safety and Health recommended exposure level for halogenated anesthetic gases of two parts per million. The employer installed a ventilation system in the procedure room after the initial sampling and results. We collected 12 full-shift samples after the ventilation system was in place. Results showed an approximate 86% decrease in isoflurane concentration.