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Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
Published on: December 27, 2014
24.9K
Immune Cell Isolation from Mouse Femur Bone Marrow.
1Division of Biosciences, The Ohio State University, Columbus, USA; Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, Columbus, USA.
Bio-Protocol
|July 22, 2016
Summary
This study presents a rapid protocol for isolating mouse bone marrow immune cells, excluding erythrocytes. The method yields approximately 8 × 10^7 cells, suitable for further immunological research.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Bone marrow is a primary site for hematopoiesis, housing diverse blood cell populations.
- Specific immune cell subsets within the bone marrow are crucial for immunological studies.
- Efficient isolation of these cells is essential for downstream applications.
Purpose of the Study:
- To develop a simple and rapid protocol for isolating mouse bone marrow immune cells.
- To exclude erythrocytes from the isolated bone marrow cell population.
- To provide a high-yield cell preparation for further analysis.
Main Methods:
- Utilizing mouse femurs as the source of bone marrow.
- Implementing a protocol for the mechanical isolation of bone marrow cells.
- Employing a method to remove erythrocytes from the cell suspension.
Main Results:
- Successful isolation of bone marrow immune cells, free of erythrocytes.
- Achieved an approximate yield of 8 × 10^7 cells in 5 ml of culture media.
- Obtained a cell concentration of 1.6 × 10^4 cells/μl.
Conclusions:
- The developed protocol offers an efficient method for obtaining enriched bone marrow immune cells.
- This technique facilitates further isolation or flow cytometric analysis of specific immune cell types.
- The protocol provides a valuable resource for immunological research requiring pure bone marrow immune cell populations.
