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Updated: Mar 17, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
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Fungal skull base osteomyelitis: Emerging microbial identification techniques.

Peter G Volsky1, Todd A Hillman1

  • 1Pittsburgh Ear Associates, Pittsburgh, Pennsylvania, U.S.A.

The Laryngoscope
|July 23, 2016
PubMed
Summary

Skull base osteomyelitis diagnosis, especially fungal infections, is challenging with traditional methods. Molecular diagnostics and biochemical assays aided in identifying the fungus, enabling successful long-term antifungal treatment.

Keywords:
Cranial basecranial basemolecular biology

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Area of Science:

  • Mycology
  • Infectious Diseases
  • Medical Diagnostics

Background:

  • Culture-based methods for identifying pathogens in skull base osteomyelitis, particularly fungi, often yield inaccurate results.
  • Skull base osteomyelitis requires accurate and timely diagnosis for effective treatment, yet diagnostic challenges persist.

Observation:

  • A patient with a 16-month history of debilitating skull base osteomyelitis caused by a fungal infection was successfully treated.
  • Diagnosis was challenging due to limitations of culture-based identification.

Findings:

  • Experimental molecular technology (Ibis T5000 universal biosensor) identified the causative fungus.
  • Biochemical assays corroborated the diagnosis of invasive fungal disease.
  • Sustained antifungal therapy led to the patient's cure.

Implications:

  • This case highlights the potential of molecular and biochemical diagnostics in managing challenging fungal skull base osteomyelitis.
  • Familiarizing otolaryngologists with these advanced diagnostic tools is crucial.
  • Further research into applying these technologies in skull base osteomyelitis is encouraged.