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Efficient positional misalignment correction method for Fourier ptychographic microscopy.

Jiasong Sun1, Qian Chen2, Yuzhen Zhang3

  • 1Smart Computational Imaging (SCI) Laboratory, Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China; Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China; sjs0808402013@163.com.

Biomedical Optics Express
|July 23, 2016
PubMed
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This summary is machine-generated.

Fourier ptychographic microscopy (FPM) uses varied light angles to achieve super-resolution. This study introduces an iterative method to correct LED position errors, enhancing image quality and reducing alignment sensitivity.

Area of Science:

  • Optics and Photonics
  • Microscopy Techniques
  • Computational Imaging

Background:

  • Fourier ptychographic microscopy (FPM) is a super-resolution imaging technique.
  • Accurate LED matrix positioning is crucial for FPM recovery quality.
  • Wide field-of-view (FOV) in FPM exhibits varying sensitivity to LED positional misalignment.

Purpose of the Study:

  • To develop an iterative method for correcting LED position errors in FPM.
  • To improve the overall quality of recovered object images in FPM.
  • To reduce the stringent requirements for LED element position accuracy in FPM alignment.

Main Methods:

  • An iterative correction method based on the simulated annealing (SA) algorithm was employed.
  • Non-linear regression and multiple low illumination NA images were used for initial global misalignment estimation.
Keywords:
(100.0100) Image processing(100.3010) Image reconstruction techniques(100.5070) Phase retrieval(110.0180) Microscopy(110.1758) Computational imaging

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  • The method was validated through simulations and experimental results.

    • Main Results:

    • The proposed iterative method effectively corrects LED position errors.
    • Improved image quality of the recovered object was demonstrated.
    • The method relaxes the precision required for LED element positioning.

    Conclusions:

    • The SA-based iterative method enhances FPM imaging performance.
    • This technique offers a more robust and less demanding approach to FPM alignment.
    • The findings contribute to more accessible and reliable super-resolution microscopy.