Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reduced Hypotonic Exposure and Sequential Membrane Cholesterol Enrichment Limit Phosphatidylserine Externalization and Enhance Circulatory Retention of Erythrocyte-Derived Microparticles.

Journal of biomedical materials research. Part A·2026

Same author

Comparative Evaluation of R134a and HFO-1234ze Cryogen Spray Cooling Using a Mouse Model With Controllable Epidermal Pigmentation.

Lasers in surgery and medicine·2026

Same author

Effects of O<sup>+</sup> and a Non-O<sup>+</sup> Blood Type, Number Concentration, and Membrane Phosphatidylserine Flipping on the Circulation Dynamics and Biodistribution of Microsized Erythrocyte-Derived Optical Particles in Mice.

ACS applied bio materials·2025

Same author

Near-infrared imaging of intraperitoneal ovarian cancer enhanced by BrCy112 in a dual mode (MR and NIR), dual-Gd nanoparticle.

Nanoscale·2025

Same author

Bicontinuous Interconnected Porous Biomaterials for Tissue Engineering and Regeneration.

Small science·2025

Same author

Fuel-driven filamentous phage nanomotors.

Nanoscale·2025

Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026

Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026

Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026

Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026

Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026

Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 17, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Combined optical micromanipulation and interferometric topography (COMMIT).

Mohammad Sarshar¹, Thompson Lu¹, Bahman Anvari¹

¹Department of Bioengineering, University of California, Riverside. 900 University Ave., Riverside, CA 92521, USA.

Biomedical Optics Express

|July 23, 2016

Summary

This summary is machine-generated.

This study introduces an all-optical platform combining optical tweezers and quantitative phase microscopy for pico-Newton force measurements and nanoscale imaging. This breakthrough enables precise, label-free analysis of biological nanostructures and their mechanical responses.

Keywords:

(120.0120) Instrumentation, measurement, and metrology (180.3170) Interference microscopy (350.4855) Optical tweezers or optical manipulation

More Related Videos

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Related Experiment Videos

Last Updated: Mar 17, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Area of Science:

Biophysics
Cell Biology
Nanotechnology

Background:

Optical tweezers are vital for pico-Newton (pN) force microscopy in mechanobiology.
Current limitations exist in concurrent nanoscale metrology for quantitative mechanical analysis.

Purpose of the Study:

To develop an integrated all-optical platform for simultaneous pN force resolution and nanoscale imaging.
To enable label-free measurement of nanostructures and nanomechanical responses in biological samples.

Main Methods:

Integration of optical tweezers with interferometric quantitative phase microscopy.
Development of an all-optical platform for parallel force and structural measurements.
Real-time micromanipulation and label-free imaging of biological samples.

Main Results:

Achieved pN force resolution in parallel with nanoscale structural imaging.
Enabled real-time, label-free measurement of nanomechanical responses.
Demonstrated a novel platform for advanced mechanobiological studies.

Conclusions:

The developed platform overcomes limitations of traditional optical tweezers by integrating imaging capabilities.
This technology opens new research avenues in understanding mechanotransduction and biological mechanics at the nanoscale.
Offers a powerful tool for quantitative analysis of biological samples.