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Intra- and interday repeatability of peripheral arterial function: suitability and potential limitations
Cristian Del Bo'1, Valeria Deon1, Marisa Porrini1
1Division of Human Nutrition, Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milan, Italy.
Microcirculation (New York, N.Y. : 1994)
|July 23, 2016
Summary
Restricted Flow Index (RHI) measured by Endo-PAT shows good repeatability between days but poor repeatability within the same day. Multiple measurements can increase RHI, particularly in those with endothelial dysfunction.
Area of Science:
- Cardiovascular physiology
- Vascular function assessment
Background:
- Assessing endothelial function is crucial for cardiovascular health.
- The Endo-PAT device is used to measure Reactive Hyperemic Index (RHI), a marker of endothelial function.
Purpose of the Study:
- To evaluate the inter- and intraday repeatability of RHI measurements using the Endo-PAT device in healthy volunteers.
- To identify potential limitations and influencing factors in RHI measurement protocols.
Main Methods:
- Interday RHI repeatability was assessed over two consecutive days in 31 male subjects.
- Intraday RHI repeatability was evaluated at baseline and after 2 and 4 hours in 16 volunteers.
- Statistical analyses included ANOVA, Bland-Altman plots, CV, CR, and ICC.
Main Results:
- Good interday RHI repeatability was observed (CV: 6.0%; CR: 0.51; ICC: 0.77).
- Intraday RHI repeatability was poor, with significant changes noted across multiple measurements (CV: 18.8%; CR: 1.26; ICC: 0.48).
- RHI significantly increased at 4 hours compared to baseline and 2 hours, indicating a potential measurement artifact.
Conclusions:
- RHI exhibits reliable interday but unreliable intraday repeatability.
- Repeated Endo-PAT measurements within a single day can artificially inflate RHI values, especially in individuals with endothelial dysfunction.
- Standardizing measurement protocols is essential to mitigate limitations associated with multiple intraday RHI evaluations.
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