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Hepatitis B virus resistance substitutions: long-term analysis by next-generation sequencing
Leandro R Jones1,2, Mariano Sede1,3, Julieta M Manrique1,2
1Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917 (C1083ACA), Buenos Aires, Argentina.
Archives of Virology
|July 23, 2016
Summary
Chronic hepatitis B virus (HBV) infection involves dominant lineages and diverse low-frequency variants. These variants may fuel viral evolution and impact treatment outcomes during antiviral therapy.
Area of Science:
- Virology
- Genetics
- Hepatology
Background:
- Chronic hepatitis B virus (HBV) infection is a global health concern.
- Understanding HBV genetic diversity is crucial for effective antiviral therapy.
Purpose of the Study:
- To investigate HBV phylogenetics and resistance-associated mutations (RAMs) during antiviral therapy.
- To characterize the dynamics of dominant and low-frequency HBV variants.
Main Methods:
- Next-generation sequencing of longitudinal samples from seven patients undergoing antiviral treatment.
- Analysis of HBV phylogenetics and RAMs.
Main Results:
- HBV populations were dominated by a few abundant lineages alongside numerous low-frequency variants.
- New RAMs emerged, and their frequencies increased during antiviral therapy.
- Low-frequency variants were present before treatment and persisted or increased.
Conclusions:
- Chronic HBV infection features dominant lineages and a reservoir of diverse low-frequency variants.
- These low-frequency variants contribute to viral genetic plasticity.
- Understanding viral dynamics is essential for predicting patient outcomes.
