Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing...

Area of Science:

• Biomarker Detection
• Biosensors
• Analytical Chemistry

Background:

• Human endoglin (CD105) is a clinically relevant biomarker.
• Accurate and rapid detection methods are crucial for clinical diagnostics.
• Existing methods may lack sensitivity or speed.

Purpose of the Study:

• To develop a sensitive and rapid magneto-actuated immunoassay for human endoglin (CD105) detection.
• To utilize amperometric detection at screen-printed carbon electrodes (SPCEs).
• To validate the method against commercial ELISA kits.

Main Methods:

• A magneto-actuated immunoassay using antibody-modified magnetic particles.
• Sandwich assay with HRP-labeled secondary antibody.
• Amperometric detection at SPCEs with hydroquinone/H2O2/HRP system.

Main Results:

• The platform detected 5 pmoles of endoglin in 30 minutes.
• Achieved high sensitivity for the clinically relevant biomarker.
• Results were statistically similar to commercial ELISA kits.

Conclusions:

• The developed magneto-actuated immunosensing platform is sensitive and rapid.
• It provides a viable alternative for quantifying endogenous endoglin in human serum.
• This method enhances clinical diagnostic capabilities for endoglin.