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Improved scaffold biocompatibility through anti-Fibronectin aptamer functionalization.

C Galli1, L Parisi2, M Piergianni3

  • 1Dep. Biomedical, Biotechnological and Translational Sciences, University of Parma, Parma, Italy; Centro Universitario di Odontoiatria, University of Parma, Parma, Italy; Istituto Materiali per l'Elettronica ed il Magnetismo IMEM-CNR, Parma, Italy.

Acta Biomaterialia
|July 25, 2016
PubMed
Summary
This summary is machine-generated.

Immobilized anti-Fibronectin aptamers on hydrogels significantly enhanced osteoblastic cell attachment, growth, and migration, improving tissue regeneration scaffolds. This aptamer coating strategy shows promise for biomaterial applications.

Keywords:
BiomimeticsCell adhesionCell culture techniquesHydrogelsOsteoblasts

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Protein adsorption critically influences cell-biomaterial interactions, impacting tissue regeneration.
  • Tailoring protein adsorption on biomaterials can guide cell behavior for enhanced regenerative outcomes.

Purpose of the Study:

  • To investigate if immobilized anti-Fibronectin aptamers can promote osteoblastic cell attachment and growth on hydrogel scaffolds.
  • To evaluate the effect of aptamer functionalization on cell behavior within 2D and 3D culture environments.

Main Methods:

  • Polyethyleneglycole diacrylate/thiolated Hyaluronic Acid (PEGDA/tHA) hydrogels were coated with anti-Fibronectin aptamers.
  • Hydrogel loading and Fibronectin binding were quantified using spectrophotometry and Bradford assay.
  • Human osteoblasts (hOBs) were cultured on functionalized hydrogels in 2D and 3D, with cell attachment, viability, morphology, and migration assessed over 10 days.

Main Results:

  • Aptamer-coated hydrogels showed increased binding of Fibronectin.
  • Significantly higher numbers of adherent and viable hOBs were observed on aptamer-functionalized hydrogels compared to controls.
  • Cells cultured on aptamer-coated scaffolds exhibited enhanced spreading, more focal adhesions, and deeper migration into the 3D hydrogel matrix.

Conclusions:

  • Immobilized anti-Fibronectin aptamers effectively promote osteoblastic cell adhesion, proliferation, and infiltration in PEGDA/tHA hydrogels.
  • This aptamer-based strategy enhances scaffold colonization and shows potential for improving biomaterials in tissue regeneration.
  • Aptamer coating offers a viable approach for functionalizing biomaterials to capture and concentrate specific bioactive molecules, improving scaffold performance.