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Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Origin assignment by multi-element stable isotopes of lamb tissues.

Shumin Sun1, Boli Guo2, Yimin Wei2

  • 1College of Food Science and Technology, Henan University of Technology, No. 1 Lianhua Rd, High-tech Industrial Development Zone, Zhengzhou 450001, PR China.

Food Chemistry
|July 25, 2016
PubMed
Summary

Stable isotope analysis of carbon, nitrogen, and hydrogen in lamb meat and wool effectively traces geographical origins. This multi-element isotope approach aids in authenticating lamb products and ensuring traceability.

Keywords:
Lamb defatted muscleOrigin assignmentStable isotopesWool

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Isotope Geochemistry

Background:

  • Geographical origin is a critical factor in lamb meat quality and consumer perception.
  • Accurate traceability of lamb products is essential for food safety and authenticity.
  • Isotopic analysis offers a potential method for geographical origin assignment in food products.

Purpose of the Study:

  • To investigate the potential of carbon (C), nitrogen (N), and hydrogen (H) stable isotope compositions in lamb meat and wool for determining geographical origin.
  • To assess the correlation between isotopic values in lamb tissues and their feed/water sources.
  • To evaluate the effectiveness of multi-element isotope analysis for lamb traceability.

Main Methods:

  • Isotope Ratio Mass Spectrometry (IRMS) was used to determine the isotopic compositions (δ(13)C, δ(15)N, δ(2)H) in lamb meat and wool samples.
  • Samples were collected from five different regions in China, representing two distinct feeding regimes.
  • Statistical analysis was employed to assess differences and correlations among isotopic values and geographical origins.

Main Results:

  • Significant regional variations in δ(13)C, δ(15)N, and δ(2)H values were observed in lamb tissues.
  • δ(13)C values showed a strong correlation with lamb feed isotopic composition (p<0.01).
  • δ(2)H values were significantly correlated with lamb drinking water isotopic composition (p<0.05).
  • Isotopic values (δ(13)C, δ(15)N, δ(2)H) in lamb muscle were highly correlated with those in wool samples.
  • A high classification accuracy of 88.9% for muscle and 83.8% for wool was achieved using a combination of C, N, and H isotopes.

Conclusions:

  • Multi-element stable isotope analysis (C, N, H) is an effective tool for identifying the geographical origin of lamb meat.
  • Both lamb muscle and wool tissues can be utilized for robust lamb traceability.
  • Isotopic fingerprinting provides a reliable method for ensuring the authenticity and provenance of lamb products.