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ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
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Hierarchically Assembled Nanofibers Created by a Layer-by-Layer Self-Assembly.

Tae-hwan Han, Eun-sook Lee, Tae-hoon Ko

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    Hierarchically assembled nanofibers were created using layer-by-layer self-assembly. Adjusting the PSS-PAA fraction and pH in PAH/(PSS-PAA) multilayer films controlled morphology, yielding unique fibrous structures at specific conditions.

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    Area of Science:

    • Materials Science
    • Nanotechnology
    • Polymer Science

    Background:

    • Layer-by-layer (LbL) self-assembly is a versatile technique for fabricating multilayer films.
    • Controlling film morphology at the nanoscale is crucial for advanced material applications.

    Purpose of the Study:

    • To investigate the influence of PSS-PAA fraction and pH on the morphology of PAH/(PSS-PAA) multilayer films.
    • To achieve controlled hierarchical assembly of nanofibers via LbL self-assembly.

    Main Methods:

    • Fabrication of multilayer films using LbL self-assembly with varying PSS-PAA fractions and pH.
    • Characterization of film morphology using atomic force microscopy (AFM).
    • Analysis of film thickness and crystal structure using X-ray reflectivity and scattering.

    Main Results:

    • Surface morphology transitioned from globular clusters to worm-like and then to fibrous structures with increasing PSS-PAA fraction.
    • Fibrous morphologies with ~50 nm diameter and narrow distribution were achieved at a PSS-PAA fraction of 0.9 and pH 3.5-4.2.
    • Film thickness per bilayer was approximately 8.6 nm, with crystal sizes increasing with bilayer number.

    Conclusions:

    • The PSS-PAA fraction and pH are critical parameters for controlling the hierarchical assembly and morphology of LbL multilayer films.
    • LbL self-assembly offers a pathway to engineer specific nanostructures like nanofibers for potential applications.