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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Mar 17, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Protein microarray applications: Autoantibody detection and posttranslational modification.

Apurva Atak¹, Shuvolina Mukherjee¹, Rekha Jain¹

¹Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.

|July 26, 2016

Summary

This summary is machine-generated.

Protein microarrays enable simultaneous study of thousands of proteins, advancing diagnostics and functional studies. This review highlights their applications in autoantibody profiling and post-translational modification (PTM) research.

Keywords:

Autoantibody profiling PTM studies Protein arrays Tissue microarrays

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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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Published on: May 4, 2012

Area of Science:

Proteomics
Genomics
Molecular Biology

Background:

DNA microarrays are limited in predicting protein structure and dynamics.
Proteins are the key effector molecules in cellular functions.
Protein microarrays offer a platform for high-throughput protein analysis.

Purpose of the Study:

To summarize protein microarray platforms.
To discuss biological and clinical applications of protein microarrays.
To highlight the role of tissue microarrays in validating proteomic findings.

Main Methods:

Review of various protein microarray technologies.
Analysis of antibody arrays for protein quantification and PTM assessment.
Discussion of protein-protein and protein-ligand interaction studies.

Main Results:

Protein microarrays facilitate simultaneous study of thousands of proteins/peptides.
Antibody arrays enable quantification of protein abundance and PTMs.
Applications include autoantibody profiling and PTM studies in disease.

Conclusions:

Protein microarrays are valuable tools in proteomics.
They offer significant potential in biological and clinical applications.
Tissue microarrays are crucial for validating findings from protein arrays.