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Updated: Mar 17, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[Correlation between MALDI-TOF Vitek-MSTM system and conventional identification methods of gastrointestinal
C Ruiz de Alegría-Puig1, A Aguirre-Quiñonero, J Agüero-Balbín
1Carlos Ruiz de Alegría-Puig, Hospital Universitario Marqués de Valdecilla-IDIVAL (Servicio de Microbiología), Avda.Valdecilla s/n Cp: 39008. Santander, Spain. carlosrdap@hotmail.com.
Objective:
Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MSTM and conventional methods for bacterial identification causing gastrointestinal infection.
Methods:
A total of 329 gastrointestinal pathogens were identified using Vitek-MSTM (v2 SARAMIS MS -ID, bioMérieux, Marcy-I´Étoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed.
Results:
The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %).
Conclusions:
Vitek-MSTM is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii.
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