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Updated: Mar 17, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Real-time selective sequencing using nanopore technology.

Matthew Loose1, Sunir Malla1, Michael Stout1

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Nature Methods
|July 26, 2016
PubMed
Summary
This summary is machine-generated.

Oxford Nanopore

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • The MinION nanopore sequencer offers potential for targeted DNA sequencing.
  • Current methods often lack specificity in selecting molecules for sequencing.

Purpose of the Study:

  • To develop and demonstrate a real-time method for selective DNA molecule sequencing.
  • To enable targeted sequencing applications using nanopore technology.

Main Methods:

  • Utilized dynamic time warping to match sequencing reads to reference sequences.
  • Implemented voltage reversal across nanopores for real-time molecule selection.
  • Developed and applied open-source Read Until software.

Main Results:

  • Successfully demonstrated real-time selective sequencing of specific genomic regions.
  • Achieved enrichment and normalization of targeted amplicon sets.
  • Validated the Read Until software for selective sequencing applications.

Conclusions:

  • Real-time selective sequencing is feasible with Oxford Nanopore MinION.
  • The Read Until software provides a powerful tool for targeted nanopore sequencing.
  • This approach enhances efficiency and specificity in genomic analyses.