Separation and molecular weight determination of prostaglandin B1 oligomers by size-exclusion chromatography

An efficient size-exclusion chromatographic method for the simultaneous separation and molecular weight (MW) determination of prostaglandin (PG) oligomers on Sephadex G-50 with borate buffer is described. The prostaglandins 15-keto-PGB1 and 16,16-dimethyl-15-keto-PGB1 were used for the synthesis of oligomers. MW determinations in the monomer to octamer range is based on the linear correlation between the partition coefficient and log (MW) of the oligomers. The essential role of the borate anion between the gel matrix and the oligomers is demonstrated.